DNA & Genetics

We offer a variety of interesting and fun science and biotech workshops for students at secondary levels.

The World of DNA

* Registration Flow and Payment Method

Genetic Traits – Bitter Genes(Course Code: BML-SD-01)

For Secondary 1-2

Duration: ~30 min

Student can learn about the basics of genetics, and some common genetic traits from a short lecture. They can check their bitter gene by phenylthiocarbamide (PTC) taste test paper. There is a single gene which codes for a protein on our tongues. PTC binds to the protein if it presents, and the students can taste the bitterness. If the protein is not present, PTC cannot bind and the student cannot taste the bitterness. Being able to taste PTC is a dominant trait.

 

 

 

Paper DNA Models(Course Code: BML-SD-02)

For Secondary 1-4

Duration: ~30 min

Student can learn about the basics of genetics, and the structure of DNA from a short lecture. They can fold a piece of paper into 3-dimentional double helical structure of a DNA molecule.

 

 

 

Extraction of DNA from Bacterial Cells(Course Code: BML-SD-03)

For Secondary 1-4

Duration: ~60 min

Student can learn about the basics of genetics, and cellular structures of prokaryotes and eukaryotes from a short lecture. They can use a simple method to extract DNA from E.coli cells, and realize DNA is tangible!

 

 

 

Agarose Gel Electrophoresis – The Principle and Applications(Course Code: BML-SD-04)

For Secondary 4-6

Duration: ~2 – 2.5 hr

Class Size: Limited to 32 students

Student can learn about the principle of agarose gel electrophoresis, and its applications as DNA Fingerprinting from a short lecture. They can set up agarose gels (optional), use micropipettors to deliver a small volume of samples into an agarose gel, and run the gel electrophoresis. It is challenging, but fun for students to observe the separation of coloured samples within a short period of time.

 

 

 

Restriction Digestion Analysis – Paper Exercise(Course Code: BML-SD-05)

For Secondary 4-6

Duration: ~15-20 min

Student can learn about the concept of restriction digestion, and its applications as DNA Fingerprinting from a short lecture. They can do a simple paper exercise to understand the concept of Restriction Fragment Length Polymorphism (RFLP).

 

 

 
 
Restriction Digestion Analysis of Lambda DNA(Course Code: BML-SD-06)

For Secondary 5-6

Duration: ~ 3.5 hr

Class Size: Limited to 32 students

Student can learn about the concept of restriction digestion, and its applications as DNA Fingerprinting from a short lecture. They can use micropipettors to deliver pre-cut DNA samples into an agarose gel, and run the gel electrophoresis. It is challenging, but fun for students to understand the concept of Restriction Fragment Length Polymorphism (RFLP) through hands-on experiment.

 

 

 
Bacterial Transformation with Green Fluorescent Protein (GFP) Gene (Course Code: BML-SD-07)

(For Secondary 5-6)

Duration: Day One: ~3 – 3.5 hrs for hand-on experiments, and

                   Day Two: ~30 min for Result Analysis and Discussion

Class Size: Limited to 32 students

Students can learn about the concept of Recombinant DNA Technology, and its applications. They can use micropipettors to deliver DNA plasmids with a gene that codes for Green Fluorescent Protein (GFP) into bacterial cells for transformation. It is an interesting and amazing hands-on experiment to “visualize” the transformed bacterial colonies with green fluorescence under UV light!

Additional charge (HK$2,000) will be posted for this experiment (Course Code: BML-SD-07)

 

 

 
 DNA Fingerprinting – Amplification of DNA Samples by Polymerase Chain Reaction (PCR) (Course Code: BML-SD-08)

For Secondary 5-6

Duration: 6-7 hours

Class Size: Limited to 32 students

Students can learn about the concept of Polymerase Chain Reaction (PCR), and its applications as DNA Fingerprinting. Students can extract their own DNA from hair or eyebrows, and amplify their DNA sample by a thermal cycler. After PCR, they use micropipettors to deliver DNA samples into an agarose gel, and run the gel electrophoresis. It is challenging, but fun for students to understand the concept of DNA Fingerprinting through hands-on experiment.

Additional charge (HK$2,000) will be posted for this experiment (Course Code: BML-SD-08)